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Because many universal bacterial primers are actually designed and optimized specifically for qPCR or NGS, it really is recommended to take into consideration probable biases ensuing from primer-specific amplification performance for particular website taxa [35] and differential primer coverage, when working with different primer sets for qPCR and NGS. In this particular examine, we chose a broadly-utilised common bacterial primer established optimized for qPCR [29] that equally targets the V3-V4 hypervariable areas as being the primer established employed for Illumina MiSeq (S1A Desk).
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